Comparison of immune responses and intestinal flora in epicutaneously sensitized BALB/c or C57BL/6 mouse models of food allergy

Cutaneous exposure to food allergens through a disrupted skin barrier is recognized as an important cause of allergy, and the cutaneous sensitized mouse model has been established investigate relevant allergic disorders. However, role different genetic backgrounds mice on immune responses upon epicutaneous sensitization largely unknown. In this study, two strains mice, i.e., BALB/c C57BL/6 were epicutaneously with ovalbumin atopic dermatitis (AD)-like lesions, followed by intragastric challenge induce IgE-mediated allergy. Allergic outcomes measured clinical signs, specific antibodies cytokines, cell subpopulations, well changes in intestinal function gut microbiota. Results showed that both exhibited typical food-allergic symptoms Th2-skewed response. The rather than fitter for establishing allergy since stronger Th2-biased response severer disruptions homeostasis observed. This study provides knowledge selecting appropriate associated AD-like lesions highlights variations mechanism underlying pathogenesis